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. 2019 Jun 13;216(8):1904–1924. doi: 10.1084/jem.20181657

Figure 8.

Figure 8.

The pronociceptive properties of anti-CII antibodies are dependent on the Fc region, glycosylation, and interaction with FcγRI in the joint. (A–C) B10.RIII mice injected with anti-CII mAb Fab fragments (n = 8) did not develop mechanical hypersensitivity (day 5; A) compared with CAIA (n = 13) and control (n = 7) mice. They also did not show reduction in total movement (B) or rearing (C; night 3, n = 8–19/group). (D) B10.RIII mice injected with EndoS-treated anti-CII mAbs did not develop mechanical hypersensitivity (n = 3/group). BL, baseline. (E–G) B10.RIII mice injected with EndoS-treated anti-CII mAb M2139 did not develop mechanical hypersensitivity (day 5; E) or display a reduction in locomotor activity (F and G; night 3, n = 6–7/group). (H–J) BALB/c mice lacking activating FcγRs in myeloid cells (KO-WT; I) developed mechanical hypersensitivity after injection of anti-CII mAbs compared with controls (WT-WT; J). In contrast, mice lacking activating FcγRs in nonmyeloid cells (WT-KO), including neurons, were protected (H; n = 8–9/group). Data are presented as mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001 compared with controls.