Table 1. List of primary antibodies used for both IHC and immunocytochemistry (ICC) assays.
| Species/Tissue | Fixation | Primary antibody | Figure |
|---|---|---|---|
| Mouse: DRG (IHC) | Fresh; PFA 10 min RT | Rabbit anti-NeuN (1:100, Alexa Fluor 488–conjugated, ABN78A4; Millipore) | Fig. 4 D; Fig. 9, B–E; Fig. S4 A |
| Human: DRG (IHC) | PFA 2 h 4°C | ||
| Mouse: DRG (IHC), DRG cultures (ICC) | Fresh; acetone 10 min 4°C | Rat anti-FcγRI (2 µg/ml, gift from M.S. Cragg) | Fig. 5, A, B, E, and H; Fig. 6 A; Fig. S2, A, B, E, and F; Fig. S3 B |
| Mouse: Skin (IHC) | PFA-perfused; 4-h postfixation PFA | ||
| Mouse: DRG (IHC) | PFA-perfused; 24-h postfixation PFA | Rabbit anti-Iba1 (1:500, 019-19741; Wako) | Fig. 5 B |
| Mouse: DRG (IHC), DRG cultures (ICC) | Fresh; acetone 10 min 4°C | FcγRIIb (2 µg/ml, gift from M.S. Cragg) | Fig. 5, C, D, F, and I; Fig. 6 A |
| Mouse: Skin (IHC) | PFA-perfused; 4-h postfixation PFA | ||
| Mouse: DRG (IHC) | Fresh; acetone 10 min 4°C | Goat anti-TrkA (1:50, AF1056; R&D Systems) | Fig. 5, D, H, and I; Fig. S2 B |
| Mouse: Sciatic nerve (IHC) | Fresh; PFA 10 min RT | ||
| Mouse: Skin (IHC) | PFA-perfused; 4-h postfixation PFA | Rabbit anti-PGP (1:500, ab37188; Abcam) | Fig. 5, E and F |
| Mouse: DRG cultures (ICC) | Acetone 10 min 4°C | Mouse anti–β-III tubulin (1:1,000, G7121(A); Promega) | Fig. 6 A |
| Mouse: DRG (IHC) | PFA-perfused; 24-h postfixation PFA | Chicken anti-Vimentin (1:500, ab24525; Abcam) | Fig. S2 A |
| Mouse: DRG (IHC) | Fresh; acetone 10 min 4°C | Rat anti-FcγRIII (2 µg/ml, gift from M.S. Cragg) | Fig. S2 C |
| Mouse: DRG (IHC) | Fresh; acetone 10 min 4°C | Rat anti-FcγRIV (2 µg/ml, gift from M.S. Cragg) | Fig. S2 D |
| Mouse: DRG (IHC) | PFA-perfused; 24-h postfixation PFA | Goat anti-FcγRI (1:300, N-19, sc-7642; Santa Cruz Biotechnology) | Fig. S3 A |
| Mouse: DRG (IHC) | PFA-perfused; 24-h postfixation PFA | Rabbit anti-FcγRI (1:50, 50086-R001; Sino Biological) | Fig. S2 G; Fig. S3 C |
| Mouse: DRG (IHC) | PFA-perfused; 24-h postfixation PFA | Goat anti-FcγRI (1:100, AF2074; R&D Systems) | Fig. S2 H; Fig. S3 D |
| Human DRG (IHC) | PFA 2 h 4°C | Mouse anti-FcγRI (1:100, hCD64, clone 10.1, MCA756g; Serotec) | Fig. 9, B and C |
| Human DRG (IHC) | PFA 2 h 4°C | Mouse anti-FcγRIIIa/b (1:100, hCD16 PE/cy7-conjugated, clone 3G8; BioLegend) | Fig. 9, D and E |
| Human DRG (IHC) | PFA 2 h 4°C | Mouse anti-FcγRIIa (1:100, hCD32a, clone IV.3; Stem Cell Technologies) | Not shown |
RT, room temperature.