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. 2019 Jun 13;216(8):1874–1890. doi: 10.1084/jem.20182151

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© 2019 Ricard et al.

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Figure 5. — Loss of endothelial fenestration in glomeruli endothelium and endocrine gland endothelium in Erk1^−/− Erk2^iEC−/− mice. (a) Transmission electron photomicrographs of kidney Erk1^−/− Erk2^iEC−/− mice reveal swollen endothelial cells (arrowheads) with loss of glomerular capillary cell fenestrations (arrows) and basement membrane (*) thickening and distortion with localized effacement of podocyte foot processes (p) and scattered occlusion of the glomerular capillary lumen (GCL) by fibrin (presumptive). ECN, endothelial cell nucleus; US, urinary space. Representative images from n = 4 mice per genotype. Scale bars, 500 nm. (b) Immunofluorescent staining of VEGFR2 (green) in glomeruli from Erk1^−/− Erk2^iEC−/− and WT mice 2 wk after tamoxifen injections. Representative images from n = 4 mice per genotype. Scale bars, 50 µm. (c) Transmission electron photomicrograph of pancreas from Erk1^−/− Erk2^iEC−/− mice reveal swollen endothelial cells (arrowheads) with loss of capillary endothelial cell fenestrations (arrows) and basement membrane (*) thickening and distortion compared with WT mice. BC, β cells; CL, capillary lumen; ECC, endothelial cell cytoplasm. Representative images from n = 4 mice per genotype. Scale bars, 500 nm. (d and e) Immunofluorescent staining of PV1 (green) in pancreas (d) and thyroid (e) in Erk1^−/− Erk2^iEC−/− and WT mice. Representative images from n = 4 mice per genotype. Scale bars, 50 µm. LI, Langerhans islet.