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. 2019 Jun 17;216(8):1891–1903. doi: 10.1084/jem.20182238

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© 2019 Fan et al.

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Figure 1. — Cleavage of CX3CL1 by β- and γ-secretases. (A and B) Myc-tagged CX3CL1 was expressed in BACE1-overexpressing HM cells in a dose-dependent manner. Two clonal stable cell lines (c1 and c2) were also selected from mouse neuroblastoma Neuro-2A cells transfected with myc-tagged CX3CL1. The myc antibody detected three C-terminal fragments: β and β′ fragments for BACE1 cleavages and the α-secretase–cleaved fragment. The arrowhead indicates nonspecific reacted bands with the myc antibody. (B) Both BACE1 and BACE2 similarly cleave CX3CL1, as demonstrated by coexpression of indicated plasmid DNA in HEK-293 cells. Inhibition of BACE activity by compound BACE IV diminished the levels of β and β′ fragments. (C) CX3CL1 was cleaved by γ-secretase, shown by inhibition of γ-secretase with the specific inhibitor DAPT to increase levels of fragments from BACE1 and α-secretase cleavages.