Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 17;216(8):1891–1903. doi: 10.1084/jem.20182238

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Fan et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 5. — Enhanced neurogenesis by CX3CL1-ct in transgenic mice. (A) Immunostaining of brain sections from BrdU pulse-labeled mice at P21 was performed using BrdU antibody. Scale bar, 30 µm. (B) Stereological quantification confirmed enhanced neurogenesis in the neurogenic niche of the SGZ of Tg-CX3cl1-ct/tTA mice. (C) Mice were BrdU-labeled starting at P11 for 5 d and then examined at 6 wk of age. Brain sections were fixed and costained with BrdU (red) and NeuN (green) antibodies. Scale bar, 30 μm. (D) Quantification of both BrdU-positive cells and mature neurons in granular cell layers in Tg-CX3cl1-ct/tTA mice and WT control littermates was conducted, and the results were plotted (n = 6 pairs; **, P < 0.01, Student’s t test). (E and F) Newly dividing cells in the SVZ were labeled for BrdU, marked with circles (E), and quantitatively compared from one in every six fixed sections (F; n = 4; ***, P < 0.001, Student’s t test). Error bars are ± SEM.