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. Author manuscript; available in PMC: 2019 Aug 6.
Published in final edited form as: Acta Biomater. 2019 Jan 17;87:88–96. doi: 10.1016/j.actbio.2019.01.034

Figure 7.

Figure 7

Live-dead assay of fibrin hydrogel containing NHDF cells 4 hours after T100 treatment. Calcein-AM (green) stains live cells and ethidium homodimer (red) stains dead cells. (A) Representative 5 by 5 field-of-view montage. (B) Zoomed in view around the crosslinked region for 6 replicates. (C) Areas surrounding (1) a T100 crosslinked region, (2) a control T100 without crosslinker, and (3) live and (4) dead controls. Note: The crosslinked region shows signal in the red channel even in cell-free systems with no ethidium homodimer.