Fig 1. Experimental design.

Twelve total male C57BL/6Kou mice at the age of 13–15 weeks were divided into 3 groups of 4 mice each, and treated by oral gavage with a 5 or 500 μg/kg dose of TCDD dissolved in corn oil or corn oil vehicle alone (10 mL/kg). Liver tissue was collected 19 h post-treatment. Affymetrix Mouse Exon 1.0 ST Arrays were used to assess RNA abundance. Data were normalized using RMA and homogeneity assessed; no outliers were detected. Alternative splicing events were predicted using an entropy base measure (ARH), and transcripts with significantly differential abundance were validated using RT-qPCR.