Fig 3. Total AQP2 protein expression in male and female WT and Atp6v1b1-/- animals.

(A) Immunoblot showing the two characteristic, core and glycosylated, bands of AQP2 in the top panel. The bottom panel shows the loading control using total protein stain with the Pierce Reversible Protein Stain kit. (B) Lane intensity quantification normalized to the protein loading control shows that at baseline, WT females had the highest total AQP2 and deficiency in B1 subunit of the V-ATPase reduces total AQP2, especially in the female animals. There was also a reduction in total protein in the B1^-/- male animals compared to their WT counterparts, though within our tested experimental conditions it was not statistically significant. Deficiency in the B1 subunit in female animals reduced the total protein content to such an extent that it became comparable to the level of protein in male animals of both genotypes.