Table 1. The peak areas of OVA in patch extracts and OVA standards.
| Analytes | Peak area | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Blank patch extracts with spiked OVA standards | OVA patch extracts with spiked OVA standarda | before applying patch to the skin | after applying patch to the skin | |||||||||||||||||
| 5.0 μg | 10.0 μg | 50.0 μg | 15.0 μg | Blank patches | Mean ± SD | OVA patches | Mean ± SD | Blank patches | Mean ± SD | OVA patches | Mean ± SD | |||||||||
| Ovalbumin | 37,779 | 146,021 | 1,029,026 | 194,795 | 4,382 | 6,430 | 5,054 | 5,289 ± 1,044 | 115,591 | 115,121 | 113,802 | 114,838 ± 927 | 5,269 | 5,225 | 4,095 | 4,863 ± 665 | 5,204 | 5,214 | 5,284 | 5,234 ± 44 |
aOVA 15.0μg +OVA patch extracts after applying to the skin: The values (peak area) is the response of absorbance at 214 nm for detection of OVA by HPLC analysis. These values (peak area) showed as mean+SD values.