Table 2. Effect of dissolving-microneedle-patch loaded OVA on splenocytes and lymphocytes proliferation and cytokine production in mice.

Splenocytes Proliferation
		mice		1	2	3	4	mean	s.d
Stimulation Index	Cont-Patch	(-)		1.00	1.00	1.00	1.00	1.00	0.0
		re-stimulation OVA(μg/ml)	50	1.82	1.28	1.11	1.22	1.36	0.3
			100	2.02	1.63	1.60	1.67	1.73	0.2
			1000	5.59	5.12	4.51	4.44	4.92	0.5
	OVA-Patch	(-)		1.00	1.00	1.00	1.00	1.00	0.0
		re-stimulation OVA(μg/ml)	50	2.15	2.39	2.53	2.64	2.43	0.2
			100	3.30	3.34	3.51	3.43	3.39	0.1
			1000	8.13	8.83	9.41	9.49	8.97	0.6
Splenocytes IL-2
		mice		1	2	3	4	mean	s.d
Cytokine (pg/ml)	Cont-Patch	(-)		-51.32	7.00	45.39	2.59	0.92	39.8
		re-stimulation OVA(μg/ml)	50	-64.39	-13.74	-20.84	-24.71	-30.92	22.8
			100	-9.87	-6.00	23.03	2.71	2.47	14.7
			1000	-31.16	4.16	20.13	23.84	4.24	25.1
	OVA-Patch	(-)		117.39	93.68	107.06	123.52	110.41	13.1
		re-stimulation OVA(μg/ml)	50	172.71	199.16	150.94	215.45	184.56	28.5
			100	271.90	169.97	216.58	261.10	229.89	46.6
			1000	321.74	304.65	184.00	312.87	280.81	64.9
Splenocytes IFN-Ɣ
		Mice		1	2	3	4	mean	s.d
Cytokine (pg/ml)	Cont-Patch	(-)		584.00	637.75	1004.00	1181.50	851.81	288.3
		re-stimulation OVA(μg/ml)	50	-109.75	385.25	1170.25	1654.00	774.94	788.2
			100	-19.75	826.50	1561.50	2062.75	1107.75	907.1
			1000	-182.25	1225.25	2376.50	2724.00	1535.88	1312.3
	OVA-Patch	(-)		-547.25	1106.50	1141.50	1147.75	712.13	839.8
		re-stimulation OVA(μg/ml)	50	1620.25	1705.25	1912.75	1941.50	1794.94	157.0
			100	1732.75	2964.00	4349.00	4715.25	3440.25	1365.5
			1000	1997.75	3054.00	6314.00	6902.75	4567.13	2408.2
Lymphocytes Proliferation
		Mice		1	2	3	4	mean	s.d
Stimulation Index	Cont-Patch	(-)		1.00	1.00	1.00	1.00	1.00	0.0
		re-stimulation OVA(μg/ml)	50	1.10	0.99	1.18	1.15	1.11	0.1
			100	1.18	1.02	1.34	1.40	1.24	0.2
			1000	2.70	1.90	3.07	3.41	2.77	0.6
	OVA-Patch	(-)		1.00	1.00	1.00	1.00	1.00	0.0
		re-stimulation OVA(μg/ml)	50	1.34	1.32	1.46	2.13	1.56	0.4
			100	1.50	1.52	1.82	2.63	1.87	0.5
			1000	4.34	4.22	5.95	11.88	6.60	3.6
Lymphocytes IL-2
		mice		1	2	3	4	mean	s.d
Cytokine (pg/ml)	Cont-Patch	(-)		134.81	117.55	115.45	129.32	124.28	9.3
		re-stimulation OVA(μg/ml)	50	104.16	113.52	106.26	106.10	107.51	4.1
			100	112.23	111.74	108.19	115.77	111.98	3.1
			1000	102.87	109.81	125.29	117.71	113.92	9.7
	OVA-Patch	(-)		135.45	120.45	124.48	137.39	129.44	8.3
		re-stimulation OVA(μg/ml)	50	150.45	206.90	157.39	256.42	192.79	49.3
			100	169.32	261.74	159.97	264.81	213.96	57.1
			1000	337.55	225.77	182.39	383.68	282.35	94.0
Lymphocytes IFN-Ɣ
		mice		1	2	3	4	mean	s.d
Cytokine (pg/ml)	Cont-Patch	(-)		580.25	631.50	795.25	817.75	706.19	118.1
		re-stimulation OVA(μg/ml)	50	495.25	532.75	591.50	691.50	577.75	85.6
			100	522.75	722.75	776.50	1156.50	794.63	264.8
			1000	374.00	662.75	997.75	1330.25	841.19	413.8
	OVA-Patch	(-)		390.25	784.00	974.00	1011.50	789.94	284.5
		re-stimulation OVA(μg/ml)	50	702.75	911.50	1541.50	1562.75	1179.63	438.6
			100	979.00	1111.50	1537.75	1851.50	1369.94	399.9
			1000	516.50	1204.00	1279.00	1916.50	1229.00	572.5