Table 3. Effect of dissolving-microneedle-patch loaded OVA on OVA-specific CD4⁺/CD8⁺ T cell response in mice.

CD8+/IFN-Ɣ+
		mice		1	2	3	4	mean	s.d
Percentage, %	Cont-Patch	(-)		5.80	10.30	11.40	13.95	10.36	3.4
		re-stimulation OVA(μg/ml)	50	7.40	10.50	15.60	14.40	11.98	3.7
			100	6.55	12.05	15.40	14.70	12.18	4.0
			1000	14.35	15.10	18.60	16.90	16.24	1.9
	OVA-Patch	(-)		10.10	9.10	9.35	23.60	13.04	7.1
		re-stimulation OVA(μg/ml)	50	14.85	12.55	11.75	30.70	17.46	8.9
			100	11.35	14.10	12.85	32.75	17.76	10.1
			1000	18.65	23.50	17.45	47.25	26.71	13.9
CD8+/IFN-Ɣ+
		mice		1	2	3	4	mean	s.d
Stimulation Index	Cont-Patch	(-)		1.00	1.00	1.00	1.00	1.00	0.0
		re-stimulation OVA(μg/ml)	50	1.28	1.02	1.36	1.05	1.18	0.2
			100	1.13	1.17	1.35	1.07	1.18	0.1
			1000	2.47	1.47	1.63	1.22	1.70	0.5
	OVA-Patch	(-)		1.00	1.00	1.00	1.00	1.00	0.0
		re-stimulation OVA(μg/ml)	50	1.47	1.39	1.30	1.25	1.35	0.1
			100	1.12	1.56	1.39	1.38	1.36	0.2
			1000	1.85	2.61	2.00	1.86	2.08	0.4
CD4+/IFN-Ɣ+
		mice		1	2	3	4	mean	s.d
Percentage, %	Cont-Patch	(-)		3.50	3.90	4.30	4.80	4.13	0.6
		re-stimulation OVA(μg/ml)	50	2.70	4.00	4.10	4.10	3.73	0.7
			100	3.60	3.80	3.90	4.40	3.93	0.4
			1000	5.90	6.40	6.80	7.20	6.58	0.7
	OVA-Patch	(-)		3.30	3.40	3.50	4.00	3.55	0.4
		re-stimulation OVA(μg/ml)	50	5.30	6.10	6.10	6.80	6.08	0.6
			100	5.60	5.80	6.00	6.10	5.88	0.2
			1000	7.10	7.50	7.50	7.70	7.45	0.3
CD4+/IFN-Ɣ+
		mice		1	2	3	4	mean	s.d
Stimulation Index	Cont-Patch	(-)		0.85	0.95	1.04	1.16	1.00	0.1
		re-stimulation OVA(μg/ml)	50	0.77	1.03	0.95	0.85	0.90	0.1
			100	1.03	0.97	0.91	0.92	0.96	0.1
			1000	1.69	1.64	1.58	1.50	1.60	0.1
	OVA-Patch	(-)		0.93	0.96	0.99	1.13	1.00	0.1
		re-stimulation OVA(μg/ml)	50	1.61	1.79	1.74	1.70	1.71	0.1
			100	1.70	1.71	1.71	1.53	1.66	0.1
			1000	2.15	2.21	2.14	1.93	2.11	0.1