Skip to main content
. 2019 Aug 6;8:e39495. doi: 10.7554/eLife.39495

Figure 6. npba is involved in female sexual receptivity.

(A, B) Various parameters in the mating behavior of npba (A) and npbb (B) single knockout females were measured and compared with wild-type females (n = 34, 33, and 31 for npba+/+, npba+/-, and npba-/- females, respectively; n = 29, 33, and 32 for npbb+/+, npbb+/-, and npbb-/- females, respectively). Blue, ocher, and beige columns represent wild-type, heterozygous knockout, and homozygous knockout females, respectively. *, p<0.05; **, p<0.01; ***, p<0.001 (Bonferroni’s post hoc test). (C, D) The latency data for npba (C) and npbb (D) single knockouts were further analyzed using Kaplan-Meier plots. Blue triangles, ocher diamonds, and beige circles represent wild-type, heterozygous knockout, and homozygous knockout females, respectively. *, p<0.05 (Gehan-Breslow-Wilcoxon test with Bonferroni’s correction). See also Figure 6—figure supplement 1.

Figure 6.

Figure 6—figure supplement 1. Genetic scheme for the npba-/-, npbb-/-, and npbwr2-/- mutant medaka.

Figure 6—figure supplement 1.

Knockout medaka deficient for npba (A, B), npbb (C, D), and npbwr2 (E, F) were generated by transcription activator-like effector nuclease (TALEN)-mediated genome editing. (A) Gene structure of npba showing the location of the deletion target site, which is enlarged to show the nucleotide sequences of the npba+/+ and npba-/- alleles. The mature Npba polypeptide-coding sequence is indicated in white letters on a black background. TALEN binding sites are underlined, and deleted nucleotides are indicated by dashes. (B) Comparison of the deduced Npba precursor protein sequences of the npba+/+ and npba-/- alleles. The mature Npba polypeptide is indicated in white letters on a black background. The altered sequence caused by a frameshift is shaded in gray. Asterisks indicate stop codons. (C) Gene structure of npbb showing the location of the deletion target site, which is enlarged to show the nucleotide sequences of the npbb+/+ and npbb-/- alleles. The mature Npbb polypeptide-coding sequence is indicated in white letters on a black background. TALEN binding sites are underlined and deleted nucleotides are indicated by dashes. (D) Comparison of the deduced Npbb precursor protein sequences of the npbb+/+ and npbb-/- alleles. The mature Npbb polypeptide is indicated in white letters on a black background. The altered sequence caused by a frameshift is shaded in gray. Asterisks indicate stop codons. (E) Gene structure of npbwr2 showing the location of the deletion target site, which is enlarged to show the nucleotide sequences of the npbwr2+/+ and npbwr2-/- alleles. TALEN binding sites are underlined and deleted nucleotides are indicated by dashes. (F) Comparison of the deduced Npbwr2 protein sequences of the npbwr2+/+ and npbwr2-/- alleles. The altered sequence caused by a frameshift is shaded in gray. Asterisks indicate stop codons.