FIGURE 8.

Reactivity of the antistreptococcal M5 peptide sera in a Western immunoblot of human cardiac myosin. The 200-kDa protein band of the purified human cardiac myosin, shown in the stained portion of the Western blot, reacted most strongly with antipeptide sera from mice immunized with the M5 peptides NT3-7, B2B3B, C1A, C1B, C2C3, and C3. Sera were tested at a 1:1000 dilution. A control antimyosin mAb CCM-52 (a gift from William Clark, Cardiovascular Research Institute, Michael Reese Hospital and Medical Center, Chicago, IL) reacted with the 200-kDa band present in our purified preparation of human cardiac myosin heavy chain. Purification of the human cardiac myosin heavy chain to homogeneity was previously described by Dell et al. (204). The Western blot confirms the data seen in the enzyme-linked immunosorbent assay with human cardiac myosin. (Reproduced from reference 32 with permission from ASM Press.)