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. Author manuscript; available in PMC: 2020 May 28.
Published in final edited form as: J Control Release. 2019 Apr 8;302:181–189. doi: 10.1016/j.jconrel.2019.04.009

Fig. 1. Short consensus repeats (SCRs) that we re used in the study:

Fig. 1

A) Schematic representation of membrane-bound complement control proteins (CCPs) CD55 and CD35, and plasma factor H. CCPs consist of multiple short consensus repeats (sushi domains). SCRs primarily responsible for decay accelerating activity are shown; B) structural model of complement convertase C3bBb forming a complex with CD55, leading to the decay; C) open view of SCR-1-2-3-4 (shown in different colors, from top to bottom) of DAF interacting with C3b (green). SCR-1 does not interact with C3b and is not needed for decay activity; D) different constructs containing single or multiple SCRs and His-tags. Soluble CD55 was obtained commercially, all other constructs were cloned and expressed as described in Methods; E) representative SDS-PAGE of the purified constructs.