SPIO NWs were incubated in sera and plasma in the presence or absence of increasing concentration of inhibitors as described in Methods. A) C3 deposition, SCR-2-3-4 in serum; B) C5a levels, SCR-2-3-4 in serum; C) C3 deposition, SCR-2-3-4 in plasma; D) C5a levels, SCR-2-3-4 in plasma; E) C3 deposition, soluble CD55 in serum; F) C3 deposition, SCR-2-3-4 and cP40 (compstatin) in plasma. Each graph shows a corresponding IC50 value above. Typical inhibition curve is shown; multiple donors are compiled in Table 1. All graphs show raw values on Y-axis. Each point is the average of 3 technical replicates.