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. Author manuscript; available in PMC: 2020 May 28.
Published in final edited form as: J Control Release. 2019 Apr 8;302:181–189. doi: 10.1016/j.jconrel.2019.04.009

Fig. 6. Uptake of SPIO NWs by leukocytes is inhibited by SCR-2-3-4.

Fig. 6

A) SPIO NWs were incubated with lepirudin blood in presence of 30 µg/mL (1.3µM) of SCR-2-3-4; B) Magnetically labeled cells were concentrated on a slide and stained with Hoechst. High-magnification images showing nuclear morphology are in Supplement. Most of the cells were neutrophils. The uptake was inhibited by SCR-2-3-4. Letters (f/m) and numbers are gender and age of human blood donors; C) C3 deposition in plasma of donors used in the study, and inhibition of the deposition by SCR-2-3-4. Individual data points of 3 technical replicates for each donor are shown; D) magnetic cells from F38 and F53 samples were eluted, diluted 10 times and analyzed with flow cytometry (forward scattering- light scattering) using the same volume of eluted material (20µL) for each sample. Regions of interest are leukocytes (right) and RBCs (lower left). Boxed in red are absolute numbers of leukocytes in 20 µL. SCR-2-3-4 inhibits the binding and uptake by ~90%, but does not affect the number of magnetically labeled RBCs. EDTA effect was variable.