KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
His-Tag Antibody	Thermo Fisher	R932-25
Chemicals
GMPCPP	Jena Biosciences	NU-405L
GTP	Jena Biosciences	NU-1012-LYO
GDP	Jena Biosciences	NU-1172
AMPPNP	Jena Biosciences	NU-214
ATP	Sigma	A2383
ADP	Sigma	A2754
Mant-ADP	Thermo Fisher	M12416
MgCl2	Quality Biological	7058629
DMSO	VWR	WN182
BRD9876	Tocris	5454
Colchicine	Sigma	C9754
Demecolcine	Sigma	D7385
Nocodazole	Sigma	M1404
Maytansinoid DM1	MedChemExpress	HY-19792
Paclitaxel	Sigma	T7191
α-casein	Sigma	C6780
Catalase	Sigma	C1345
Glucose Oxidase	Sigma	G6641
1H,1H,2H,2H-Perfluorodecyltrichlorosilane	Abcam	L16584
β-mercaptoethanol	Sigma	M6250
NeutrAvidin	Thermo Fisher	31000
Methyl cellulose	Sigma	M0512
Bacterial and Virus Strains
E. coli, DH5α	NEB	C2987I
E. coli, BL21(DE3)	NEB	C2527I
Critical Commercial Assays
EnzChek	Thermo Fisher	E6646
Q5 Site-directed Mutagenesis kit	NEB	E0554
Recombinant DNA
Eg5-GFP	[45]	N/A
KHC-GFP	[45]	N/A
Eg5-406	[21]	N/A
KHC-406	[36]	N/A
Eg5M	[22]	N/A
KHCM	This study	N/A
KLP67AM	This study	N/A
Eg5swap-GFP	This study	N/A
KHCswap-GFP	This study	N/A
Eg5swap	This study	N/A
KHCswap	This study	N/A
Software and Algorithms
MATLAB	Mathworks	https://www.mathworks.com
ImageJ	NIH	https://imagej.nih.gov/ij/
Micro-Manager	Vale Lab	https://micro-manager.org/
MetaVue 6.3	Molecular Devices	https://de.moleculardevices.com/
Clustal Omega	EMBL-EBI	https://www.ebi.ac.uk/Tools/msa/clustalo/
Soft MaxPro 5.4.6	Molecular Devices	https://www.moleculardevices.com/
Pro-Data SX/Viewer	Applied Photophysics	https://www.photophysics.com/
RFPC 5301	Shimadzu Scientific	https://www.ssi.shimadzu.com/support/
OriginLab Pro 9.1	OriginLab	https://www.originlab.com/
UCSF Chimera	UCSF RBVI	https://www.cgl.ucsf.edu/chimera/