Figure 7. IFN-mediated negative feedback shapes in vivo inflammatory responses in a pathogen-specific manner.
WT or Ifnar1-/- littermate mice were infected intranasally with 5 × 107 CFU of P. aeruginosa or S. aureus, as indicated. At 2 and 6 hr after infection, mice were sacrificed and BAL was collected for quantification of cytokines by CBA (A-C) and neutrophils by flow cytometry (D). Data are pooled from two independent experiments, with a total of 6 mice per experimental group. Neutrophil numbers were normalized to the mean of WT P. aeruginosa infected mice for each independent experiment. The mean values for PBS treated control mice are shown as dotted lines.
Figure 7—figure supplement 1. Lung infection with P. aeruginosa and S. aureus.
WT or Ifnar1-/- littermate mice were infected intranasally with 5 × 107 CFU of P. aeruginosa or S. aureus, and mice were sacrificed at 2- and 6 hr post-infection. CFU were determined by plating dilutions of homogenized whole lungs. IL-10 was quantified from BAL using CBA. Representative flow cytometry gating shows CD11b+Ly6G+ neutrophils shown in Figure 7D and SiglecF+CD11c- eosinophils shown below. Data are shown for one of two experiments; IL-10 was not detected above baseline in the other experiment.