Author response image 1. Model simulations of ML277 effects by 2X k₁ and 1/2X β₃.

(A) Model simulations of KCNQ1 activation currents before (black) and after (blue) adding ML277. (B) Five-state Markov model to show the two open states gating processes of KCNQ1 channel. α and β are voltage-dependent transitions. k_1-4 are VSD-pore coupling rates (constant). 2X K₁ and 1/2X β₃ can mimic the ML277 effects on KCNQ1 channel. (C-D) G–V relationships of simulated KCNQ1 before (black) and after (blue) adding ML277. Data points were fitted with a Boltzmann function.