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. 2019 Aug 6;8:e44215. doi: 10.7554/eLife.44215

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© 2019, Manenschijn et al

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Figure 8. — (A) and (B): Gallery of light microscopy (top) and electron tomography (bottom) images of invaginations (A) and vesicles (B) found in myo5Δ Sla1-EGFP Abp1-mCherry cells. The correlated fluorescent spots are indicated by arrows. Note that in (B) the same fluorescent spot correlated with two vesicles. The color of the border corresponds to the markers that were present in the endocytic spot (yellow: Sla1-EGFP + Abp1 mCherry, red: only Abp1-mCherry). Scalebars: 2 μm in light microscopy images, 100 nm in electron tomogram slices. (C) Diameter of the invagination tip. Only invaginations longer than 40 nm were included. WT data (green) from Kukulski et al. (2012a). **: p≤0.01.

Figure 8—figure supplement 1. — (A) and (B): Gallery of light microscopy (top) and electron tomography (bottom) images of invaginations (A) and vesicles (B) found in myo5Δ Sla1-EGFP Abp1-mCherry cells. The correlated fluorescent spots are indicated by arrows. Note that in (B) the same fluorescent spot correlated with two vesicles. The color of the border corresponds to the markers that were present in the endocytic spot (yellow: Sla1-EGFP + Abp1 mCherry, red: only Abp1-mCherry). Scalebars: 2 μm in light microscopy images, 100 nm in electron tomogram slices. (C) Diameter of the invagination tip. Only invaginations longer than 40 nm were included. WT data (green) from Kukulski et al. (2012a). **: p≤0.01.