Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) | Mpc1flox/flox, C57BL/6J | Gray et al., 2015 | ||
| Genetic reagent (Mus musculus) | Myogenin-Cre, C57BL/6J | Li et al., 2005 | Gift from Dr. Eric Olsen | |
| Genetic reagent (Mus musculus) | HSA-MerCreMer, C57BL/6J | McCarthy et al., 2012 | Gift from Dr. Karyn Esser | |
| Antibody | Rabbit monoclonal anti-MPC1 | Proteintech | S4154-2 | Gift from Dr. Brian Finck; (1:1000) |
| Antibody | Rabbit monoclonal anti-MPC2 (D4I7G) | Cell Signaling Technology | #46141, RRID:AB_2799295 | (1:1000) |
| Antibody | Rabbit monoclonal anti-VDAC (D73D12) | Cell Signaling Technology | #4661, RRID:AB_10557420 | (1:1000) |
| Antibody | Mouse monoclonal anti-Actin (AC-15) | Sigma | #A5441, RRID:AB_476744 | (1:10000) |
| Antibody | Rabbit monoclonal anti-Tubulin (DM1A) | Cell Signaling Technology | #3873S, RRID:AB_1904178 | (1:1000) |
| Antibody | Rabbit monoclonal anti-HSP90 | Cell Signaling | #4874, RRID:AB_2121214 | (1:1000) |
| Antibody | Total OXPHOS cocktail | Abcam | ab110413, RRID:AB_2629281 | (1:1000) |
| Antibody | Mouse monoclonal anti-AMPKα (F6) | Cell Signaling Technology | #2793, RRID:AB_915794 | (1:1000) |
| Antibody | Rabbit monoclonal anti-pAMPKα (Thr172) (40H9) | Cell Signaling Technology | #2535, RRID:AB_331250 | (1:1000) |
| Antibody | Rabbit monoclonal anti-AKT (pan) (11E7) | Cell Signaling Technology | #4685, RRID:AB_2225340 | (1:1000) |
| Antibody | Rabbit polyclonal anti-pAKT (Ser473) | Cell Signaling Technology | #9271, RRID:AB_329825 | (1:1000) |
| Antibody | Mouse monoclonal anti-PDH-E1α (D6) | Santa Cruz Biotechnology | #SC-377092, RRID:AB_2716767 | (1:1000) |
| Antibody | Rabbit polyclonal anti-pPDH-E1α (Ser232) | Millipore Sigma | #AP1063, RRID:AB_10616070 | (1:1000) |
| Antibody | Rabbit monoclonal anti-GAPDH (D16H11) | Cell Signaling Technology | #5174, RRID:AB_10622025 | (1:20000) |
| Antibody | Goat anti-Mouse Dylight 800 | ThermoFisher | SA5-10176, RRID:AB_2556756 | (1:10000) |
| Antibody | Donkey anti-Rabbit DyLight 680 | ThermoFisher | SA5-10042, RRID:AB_2556622 | (1:5000) |
| Antibody | Goat anti-Rabbit DyLight 800 | ThermoFisher | #35571, RRID:AB_614947 | (1:10000) |
| Sequence-based reagent | 36b4 | Forward: 5'-CGTCCTCGTTGGAGTGACA | Reverse: 5'-CGGTGCGTCAGGGATTG | |
| Sequence-based reagent | Mpc1 | Forward: 5'-AACTACGAGATGAGTAAGCGGC | Reverse: 5'-GTGTTTTCCCTTCAGCACGAC | |
| Sequence-based reagent | Mpc2 | Forward: 5'-CCGCTTTACAACCACCCGGCA | Reverse: 5'-CAGCACACACCAATCCCCATTTCA | |
| Sequence-based reagent | Cpt1b | Forward: 5'-GGTCCCATAAGAAACAAGACCTCC | Reverse: 5'-CAGAAAGTACCTCAGCCAGGAAAG | |
| Sequence-based reagent | Hadha | Forward: 5'-TGGATGTGGATGACATTGCT | Reverse: 5'-GGGGAAGAGTATCGGCTAGG | |
| Sequence-based reagent | Echs1 | Forward: 5'-CTTCACTGTAAGGGCAGGTG | Reverse: 5'-CTTGAGTTGGGAATCAGCAG | |
| Commercial assay or kit | FGF21 ELISA kit | ThermoFisher | NC9903102 | |
| Commercial assay or kit | Glucose Assay Kit | Sigma-Aldrich | HK20 | |
| Commercial assay or kit | High-Capacity cDNA Reverse Transcription kit | Applied Biosystems | 4368814 | |
| Commercial assay or kit | Infinity Cholesterol Reagent | Thermo Scientific | TR13421 | |
| Commercial assay or kit | Infinity Triglyceride Reagent | Thermo Scientific | TR22421 | |
| Commercial assay or kit | Leptin ELISA kit | R and D Systems | MOB00 | |
| Commercial assay or kit | Serum Ketone Kit | Wako Diagnostics | 415–73301, 411–73401, 412–73791 | |
| Commercial assay or kit | Serum NEFA kit | Wako Diagnostics | 999–34691, 995–034791, 991–34891, 993–35191, 276–76491 | |
| Commercial assay or kit | Ultra-sensitive Mouse Insulin ELISA kit | Crystal Chem | 90080 | |
| Commercial assay or kit | Insulin chemiluminescence ELISA | American Laboroatory Products | #80-INSMR-CH01 | |
| Chemical compound, drug | [1–14C]−2-deoxy-D-glucose | Perkin Elmer | NEC495001MCSBF3 | |
| Chemical compound, drug | α-Cyano-4-hydroxycinnamic acid (CHC) | Sigma-Aldrich | 476870 | |
| Chemical compound, drug | 13C3-Sodium Pyruvate (99%) | Cambridge Isotope Laboratories | 142014-11-17 | |
| Chemical compound, drug | 13C3-Sodium-L-Lactate (98%) | Cambridge Isotope Laboratories | CLM-1579 | |
| Chemical compound, drug | 14C-Palmitic acid | Perkin Elmer | NEC075H250UC | |
| Chemical compound, drug | 14C-Sodium Pyruvate | Perkin Elmer | NEC256050UC | |
| Chemical compound, drug | Antimycin A | Sigma-Aldrich | A8674 | |
| Chemical compound, drug | D-[3-3H]-glucose | Perkin Elmer | NET331C001MC SBF3 | |
| Chemical compound, drug | Insulin | Novo Nordisk | Novolin R | |
| Chemical compound, drug | Deoxy-D-glucose 2-[1,2-3H(N)] | Perkin Elmer | NET328A001MC | |
| Chemical compound, drug | Ensure | Abbott | Vanilla-57g | |
| Chemical compound, drug | FCCP | Sigma-Aldrich | C2920 | |
| Chemical compound, drug | High Fat Diet 60%Kcal from fat (HFD) | Research Diets Inc | D12492 | |
| Chemical compound, drug | Low Fat Diet 10% Kcal from fat (control diet, NFD) | Research Diets Inc | D12450J | |
| Chemical compound, drug | Normal Chow/Teklad Global Soy Protein-Free Extruded Rodent Diet Irradiated (NCD) | Envigo | 2920X | |
| Chemical compound, drug | Oligomycin A | Sigma-Aldrich | 75351–5 MG | |
| Chemical compound, drug | Rotenone | Sigma-Aldrich | R8875-1G | |
| Chemical compound, drug | Tamoxifen | Sigma-Aldrich | T5648-1G | |
| Chemical compound, drug | Triolein, [9,10-3H(N)] | Perkin Elmer | NET431001MC | |
| Chemical compound, drug | UK-5099 | ThermoFisher | 418610 | |
| Chemical compound, drug | Acetyl coenzyme A sodium salt | Sigma-Aldrich | A2056-10MG | |
| Chemical compound, drug | 3H-Acetyl-CoA | Perkin Elmer | NET290050UC | |
| Chemical compound, drug | Malonyl coenzyme A lithium salt | Sigma-Aldrich | M4263-5MG | |
| Chemical compound, drug | Purified chicken fatty acid synthase | David Thomson Lab, BYU | ||
| Chemical compound, drug | Protease Arrest | G Biosciences | 786–437 | |
| Other | Accupulser | World Precision Instrument | A310 Accupulser | |
| Other | Body Composition Analyzer | Bruker | LF50-BCA Analyzer | |
| Other | Callipers | MSC Industrial Supply | 35518166 | |
| Other | Glucose Meter | LifeScan | Onetouch ulta mini | |
| Other | Glucose Strips | Diabetic Express | Feb-67 | |
| Other | Headstage | Axon Instruments | CV 203BV | |
| Other | Integrating patch clamp | Axon Instruments | Axopatch 200B | |
| Other | Lactate Meter | Nova Biomedical | Lactate plus meter | |
| Other | Lactate Strips | Nova Biomedical | NC0071872 | |
| Other | Low noise data acquisition system | Axon Instruments | Axon digidata 1550 | |
| Other | Low noise data acquisition system | Axon Instruments | Axon digidata 1440A | |
| Other | Microvette blood collection tubes | Sarstedt Inc | NC9141704 | |
| Other | Ohaus Hand-Held Scales | ThermoFisher | S65222 | |
| Other | Promethion Cages | Sable Systems International | Promethion Line | |
| Other | Rodent Treadmill | Columbus Instruments | Exer 3/6 Treadmill | |
| Other | Stimulus Isolater | World Precision Instrument | Stimulus Isolater | |
| Other | Western Blot Imager | Li-Cor | Odyssey CLx | |
| Other | Platform | Aurora Scientific | 809B | |
| Other | Force tranducer | Aurora Scientific | 305C | |
| Other | Refridgerated/Heated bath circulator | Thermo Fisher | 6200 R20F | |
| Other | XF96 4-port FluxPak with PET Microplates | Seahorse Bioscience | 102416–100 | |
| Software/tool | Sigmaplot | Sigmaplot | RRID:SCR_003210 | |
| Software/tool | Excel | Microsoft | RRID:SCR_016137 |