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. Author manuscript; available in PMC: 2020 Aug 20.
Published in final edited form as: Gene. 2019 Jun 6;710:246–257. doi: 10.1016/j.gene.2019.06.005

Fig. 6.

Fig. 6.

Identification of biological processes related to tumor progression and metastasis reveled by physical interaction network analysis of proteins encoding for genes targeted by EVs miRNAs differentially expressed for metastatic osteosarcoma cells. Target prediction for selected EVs miRNAs: miR-143-3, miR181a-5p, miR-148a-5p, and miR-21-5p, differentially overexpressed in the metastatic SAOS2 osteosarcoma cell line, was carried out using TargetScan. 4-Set venn diagram show number of genes (red circle) that are targeted for multiple EVs miRNAs (A). The resulting 75 multiple miRNA target genes were further analysed with STRINGv11 (http://string-db.org/) to identify functional interaction networks containing 31 of these target genes (B) and a high confidence core network of six target genes (C). The gene network components showed in C (D) as well as gene target for each EVs miRNA (E) were analyzed with David 6.8 to identify specific pathways and biological processes functionally linked to tumor progression and metastasis.