KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| CD45 | Biolegend | Cat# 109804; RRID: AB_313441 |
| Ter119 | Biolegend | Cat # 116204; RRID: AB_313704 |
| CD31 | Biolegend | Cat # 102404; RRID: 312889 |
| CD51 | BD Biosciences | Cat # 740062 |
| Seal | Biolegend | Cat # 108112; RRID: AB_313349 |
| CD140a | eBioscience | Cat # 25–1401–82; RRID: AB_2573400 |
| CD276 | Biolegend | Cat # 124508; RRID: AB_1279206 |
| CX3CL1 | R&D Systems | Cat # FAB571G–025 |
| CD59a | Miltenyi Biotec | Cat # 130–104–106 |
| ENPP1 | Biolegend | Cat # 149207; RRID: AB_2565483 |
| CD44 | Biolegend | Cat # 103028; RRID: 830785 |
| ICAM | Miltenyi Biotec | Cat # 130–104–218 |
| CD49a | Miltenyi Biotec | Cat # 130–107–636 |
| CD56 | Novus Biologicals | Cat # FAB7820N |
| CD55 | Biolegend | Cat# 131805; RRID: 1279263 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Collagenase/Dispase | Sigma-Aldrich | Cat# 11097113001 |
| Critical Commercial Assays | ||
| Osteogenic media | STEMCELL Technologies | Cat# 05504 |
| Adipogenesis media | STEMCELL Technologies | Cat# 05503 |
| Chondrocyte differentiation | GIBCO | Cat# A1007101 |
| shRNA - TF501493 (Nr4a3) | Origene | Mouse, 4 unique 29-mer shRNA constructs in retroviral RFP vector |
| shRNA-TF501290(Maff) | Origene | 4 unique shRNA |
| shRNA - TF500537 (Dlx3) | Origene | 4 unique shRNA |
| shRNA - TF513785 (Creb3l3) | Origene | 4 unique shRNA |
| shRNA-TF511428 (Mef2c) | Origene | 4 unique shRNA |
| shRNA-TF510502 (Runx2) | Origene | 4 unique shRNA |
| shRNA-TF511411 (Sox9) | Origene | 4 unique shRNA |
| shRNA - TF500345 (Cebpb) | Origene | 4 unique shRNA |
| shRNA - TF500346 (Cebpd) | Origene | 4 unique shRNA |
| ShRNA-TF514041 (Sp7) | Origene | 4 unique shRNA |
| shRNA - TF510219 (Pparg) | Origene | 4 unique shRNA |
| Deposited Data | ||
| Sequencing Reads, feature-barcode matrices | GEO | GSE132151 |
| Experimental Models: Organisms/Strains | ||
| C57BL/6J | Jackson Laboratory | Cat# 000664 |
| Mouse: Prrx1-Cre | Jackson Laboratory | Cat# 005584 |
| Mouse: Ocn-Cre | Jackson Laboratory | Cat# 019509 |
| Mouse: AdipoQ-Cre | Jackson Laboratory | Cat# 010803 |
| Mouse: tdTom | Jackson Laboratory | Cat# 007914 |
| Software and Algorithms | ||
| Velocyto | (La Manno et al., 2018) | http://velocyto.org/ |
| dropEst | (Petukhovet al., 2018) | https://github.com/hms-dbmi/dropEst |
| Monocle2 | (Qiu etal.,2017) | http://cole-trapnell-lab.github.io/monocle-release/ |
| SPRING | (Weinrebetal., 2018a) | https://github.com/AllonKleinLab/SPRING_dev |
| PBA | (Weinrebetal., 2018b) | https://github.com/AllonKleinLab/PBA |
| Scrublet | (Wolocketal., 2019) | https://github.com/AllonKleinLab/scrublet |
| GSEA | (Subramanian et al., 2005); (Mootha et al., 2003) | http://software.broadinstitute.org/gsea/index.jsp |
| Other | ||
| MEM alpha | Fisher Scientific | Cat# 41–061–037 |
| Penicillin/Streptomycin | Fisher Scientific | Cat# BW17–602E |
| Fetal Bovine Serum | Denville | Cat# FB5001H |
| Anti-anti | Fisher Scientific | Cat# 15–240–062 |