KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| LHX1 | R&D | MAB2725, RRID:AB_2135636 |
| MAFB | R&D | MAB3810, RRID:AB_2137675 |
| PAX8 | Abcam | ab189249, |
| KRT8/18 | DSHB | Troma-1 |
| NPHS2 | abcam | ab50339, RRID:AB_882097 |
| SYNPO | R&D | MAB8977 |
| TGFBR3 | R&D | AF-242-PB, RRID:AB_354417 |
| ANXA1 | Cell Signaling | 32934 |
| ARMH4/C14orf37 | Sigma Aldrich | HPA001789, RRID:AB_1078328 |
| WT1 | abcam | ab89901, RRID:AB_2043201 |
| CUBN | Santa Cruz | sc-20607, RRID:AB_2230037 |
| CDH1 | Biosciences | 610182, RRID:AB_397581 |
| PDGFRB | Abcam | ab32570, RRID:AB_777165 |
| VEGFR2 | Cell Signaling | 2479, RRID:AB_2212507 |
| GFRA3 | R&D | AF670, RRID:AB_2110447 |
| PAX2 | R&D | AF3364, RRID:AB_10889828 |
| F3 | R&D | AF2339, RRID:AB_442150) |
| Plvap | BioRad | MCA2539GA, RRID:AB_931734 |
| GATA3 | R&D | AF2605, RRID:AB_2108571 |
| PECAM1 | BD Pharmingen | BDB550274 |
| EHD3 | Novus Biologicals | NBP2-31896 |
| FOXC2 | R&D | AF6989, RRID:AB_10973139 |
| Fluorescent in situ hybridization probes | ||
| MAFB | Advanced Cell Diagnostics, Inc. | 400801-C2 |
| RPS21 | Advanced Cell Diagnostics, Inc. | 511381-C3 |
| OLFM3 | Advanced Cell Diagnostics, Inc. | 549051-C3 |
| PLA2R1 | Advanced Cell Diagnostics, Inc. | 524581 |
| TNNT2 | Advanced Cell Diagnostics, Inc. | 518991-C3 |
| COL4A3 | Advanced Cell Diagnostics, Inc. | 461861 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| CHIR99021 | Sigma Aldrich | SML1046-25MG |
| Recombinant Human/Mouse/Rat Activin A Protein | R&D | 338-AC-050 |
| Recombinant Human FGF-basic (154 a.a.) | PeproTech | 10771-940 |
| Recombinant Human FGF-9 Protein | R&D | 273-F9 |
| Recombinant Human VEGF 165 Protein | R&D | 293-VE-010 |
| StemFit Basic02 | Ajinomoto | Basic02 |
| Y27632 | StemCell Technologies | 72302 |
| Accutase | StemCell Technologies | 07920 |
| Cas9 Protein with NLS | PNA Bio | CP02 |
| mTeSR | StemCell Technologies | 85850 |
| Critical Commercial Assays | ||
| Chromium Single Cell 3’ Library & Gel Bead Kit v2 | 10X Genomics | 120237 |
| Chromium Single Cell A Chip Kit | 10X Genomics | 120236 |
| Chromium i7 Multiplex Kit | 10X Genomics | 120262 |
| RNeasy | QIAGEN | 74104 |
| RNAscope® Multiplex Fluorescent Reagent Kit v2 | Advanced Cell Diagnostics, Inc. | 323100 |
| KAPA Stranded mRNA-Seq Kit | Kapa Biosystems | KK8420 |
| Deposited Data | ||
| Bulk RNA-Seq of human fetal renal corpuscles and whole kidney (minus renal corpuscles) | Gene Expression Omnibus | GSE127344 |
| Bulk RNA-Seq of organoids collected at various differentiation timepoints | Gene Expression Omnibus | GSE124392 |
| scRNA-Seq of human fetal kidneys and organoids (day 16 and day 28) | Gene Expression Omnibus | GSE124472 |
| Experimental Models: Cell Lines | ||
| H9hESC | WiCell | WA09 |
| Experimental Models: Organisms/Strains | ||
| Immunocompromised mice | The Jackson Laboratory | NOD.CB17-Prkdc<SCID>/J, RRID:MGI:5652139 |
| Software and Algorithms | ||
| Seurat | Satija et al., 2015 | http://satijalab.org/seurat/ |
| Similarity Weighted Nonnegative Embedding (SWNE) | Wu et al., 2018 | https://github.com/yanwu2014/swne |
| Monocle 2.0 | Qiu et al., 2017 | http://cole-trapnell-lab.github.io/monocle-release/ |
| Cell Ranger 2.1 | 10X Genomics | https://support.10xgenomics.com/single-cell-gene-expression/software/downloads/latest |