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. 2019 Aug 6;8:e46703. doi: 10.7554/eLife.46703

Figure 2. ZTF-11 is a direct transcriptional target of proneural bHLH genes.

(A) ztf-11 transcriptional reporter expression in head neurons is strongly decreased in E/daughterless homolog hlh-2(tm1768) hypomorphic mutant. Left, representative confocal images of ztf-11 transcriptional reporter in wt or hlh-2. Synchronized early L1 animals were used in the experiment. Right, transcriptional activity was measured in total GFP intensity within each animal’s head (white dashed outlines), n = 20 and 22, respectively. Error bars, S.D. ****p<0.0005, Student’s t-test two-tailed. See Figure 2—source data 1 for numerical data. (B) E-boxes are required for ZTF-11 transcriptional reporter expression. wt, wild-type ztf-11 promoter driving GFP and mCherry reporters. ΔE-box, 8 E-box sequences (CANNTG) nearest to ztf-11 tss were mutated to ACnnAG for GFP driving promoter, mCherry expression is under wild-type ztf-11 promoter as expression level control. Synchronized early L1 animals were used in the experiment. Left, representative confocal images. Right, relative intensities between GFP and mCherry within each animal’s head (white dashed outlines) were quantified. n = 10 each. Error bars, S.E.M. **p<0.05, Student’s t-test two-tailed. See Figure 2—source data 2 for numerical data.

Figure 2—source data 1. Raw fluorescence intensity values of ZTF-11 transcriptional reporter in wild-type or hlh-2(tm1768) animals.
elife-46703-fig2-data1.xlsx (8.7KB, xlsx)
DOI: 10.7554/eLife.46703.009
Figure 2—source data 2. Normalized fluorescence intensity values of wild-type or ∆E-box transcriptional reporters.
elife-46703-fig2-data2.xlsx (8.3KB, xlsx)
DOI: 10.7554/eLife.46703.010

Figure 2.

Figure 2—figure supplement 1. Expression pattern of ZTF-11 transcriptional reporter.

Figure 2—figure supplement 1.

ZTF-11 transcriptional reporter (magenta) colocalizes with endogenous ZTF-11::GFP (green) in the embryo. Control ZTF-11 promoter fusion construct used in Figure 2 was used to generate ZTF-11 transcriptional reporter.