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. Author manuscript; available in PMC: 2019 Aug 6.
Published in final edited form as: Cell Rep. 2019 Jul 30;28(5):1307–1322.e8. doi: 10.1016/j.celrep.2019.06.079
CELF1 KO and Rescue
sgRNA 5′ – GGACCTGATGGCCTGAGCCG – 3′
Genomic DNA 5′ – GGA CCT GAT GGC CTG AGC CGA GGT– 3′
Rescue cDNA 5′ – GGA CCT GAT GGC CTG AGC CGA GCT – 3′
Rescue cDNA sequence surrounding the PAM site mutation (in red, sgRNA sequence in yellow) GACATCCGAGTCATGTTCTCTTCGTTTGGACAGATTGAAGAATGCCGGATATTGCGGGGACCTGATGGCCTGAGCCGAGCTTGTGCATTTGTGACTTTTACAACAAGAGCCATGGCACAGACGGCTATCAAGGCAATGCACCAAGCACAGACCAT