Figure 5.

IgE cross-reactivity between Ara h 2 and Ara h 6 investigated with a competitive fluid-phase assay. A, schematic principle of fluid-phase assay (see Methods); B, an example of serum displaying co-sensitization with very low IgE-cross-reactivity to Ara h 2 and Ara h 6 and the corresponding capacity of 2S-albumins to trigger mast cell degranulation; C, an example of serum displaying high IgE-cross-reactivity between Ara h 2 and Ara h 6; D, an example of serum displaying IgE reactivity to Ara h 6 (as detected by solid-phase assay) only due to cross-reactive IgE specific to Ara h 2 and the corresponding capacity of 2S-albumins to trigger mast cell degranulation.