KLF12 is preferentially expressed in mature mouse NK cells. Quantitative PCR of Klf12 expression in (A) sorted splenic populations defined as CD4+ T cells: TCRβ+NK1.1−CD8−CD4+CD25−; CD8+ T cells: TCRβ+NK1.1−CD4−CD8+CD25−; CD19+ B cells: TCRβ−NK1.1−CD19+; NK cells: TCRβ−NK1.1+, and (B) sorted NK cell developmental stages in bone marrow defined as NKP: CD3−CD8−CD19−Ter119−Gr1−NK1.1−DX5−CD122+, iNK: CD122+NK1.1+DX5−, mNK: CD122+NK1.1+DX5+. Developmental stages in spleen defined as iNK: TCRβ−NK1.1+CD27+CD11b−, DP: TCRβ−NK1.1+CD27+CD11b+, mNK: TCRβ−NK1.1+CD27−CD11b+. Data are representative of 2 experiments (n = 3 mice/experiment).