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. Author manuscript; available in PMC: 2020 Aug 15.
Published in final edited form as: J Immunol. 2019 Jul 12;203(4):981–989. doi: 10.4049/jimmunol.1900396

FIGURE 2.

FIGURE 2.

Targeted disruption of the Klf12 locus. (A) Schematic of the targeting strategy into the Klf12 locus to generate Klf12 conditional knockout mice after excision of the lacZ and neomycin cassettes by Flippase recombination. (B) Southern blot of gDNA from selected ES cell clones digested with EcoRV and hybridized to a probe indicated in (A). The 7.68 kb band is the WT allele and the 6.44 kb band is the targeted allele. (C) Long range PCR of genomic (g)DNA from heterozygous (lane 1) and WT (lane 2) mice amplifying the 3’ arm of the targeting vector with primers indicated in (A). The 5.7 kb band is the targeted allele. (D) PCR of mouse gDNA from indicated genotypes. (E) Quantitative PCR amplifying exons 2–3 (left) or exons 5–6 (right) of Klf12. (F) PCR sequence of Klf12 cDNA from WT or Klf12F/F β-actin Cre+ mice. The premature stop codon is highlighted in grey. (E-F) Data are representative of n = 3 mice/genotype.