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. Author manuscript; available in PMC: 2019 Aug 7.
Published in final edited form as: Yeast. 2019 Jun 27;36(8):495–518. doi: 10.1002/yea.3395

Figure 5. Flow cytometry transcription assays.

Figure 5

Cells gated based on forward scatter, side scatter and fluorescence (see Figure A3, a–c) were used for (a) dose-response experiments done with wild-type cells in wells that were either untreated or coated with BSA and analyzed either immediately (live cells) or after chemical fixation. (b) Dose-response experiments done with wild-type, gpa1G302S, and bar1Δ cells in BSA-coated wells were analyzed immediately or after chemical fixation. Data were fitted using the sigmoidal dose-response in Prism (GraphPad). Data are presented as mean ± standard deviation, N = 4.