Table 1.
The level of spontaneous dysfunctional (DDR+) telomeres in rat embryonic fibroblasts (rEFs) and cultured in vitro pluripotent stem cells of rat: embryonic stem cells (rESCs) and induced pluripotent stem cells (riPSCs).
| Cell lines; passage | No. of metaphases in % with Meta-TIFs | Avr. No. of Meta-TIFs per metaphase | No. of metaphases in % with 5 and more Meta-TIFs of chromosome type | |||
|---|---|---|---|---|---|---|
| Total | Chromatid type | Chromosome type | Total | Chromosome type | ||
| rEFs | ||||||
| RWF1, 6 | 100 | 57 | 48 | 3.60 (2–10) | 0.68 | 0 |
| RWF1, 29 | 100 | 59 | 71 | 2.66 (1–10) | 1.31 | 12 |
| RWM1, 5 | 100 | 72 | 18 | 4.53 (2–17) | 0.07 | 3 |
| RWM1, 25 | 72 | 72 | 65 | 3.83 (0–18) | 1.86 | 9 |
| RNFM1; 9 | 95 | 81 | 28 | 1.46 (0–8) | 0.54 | 4 |
| RNFM1, 23 | 92 | 72 | 32 | 3.79 (0–15) | 1.05 | 11 |
| RNFF1, 9 | 93 | 80 | 31 | 1.46 (0–5) | 0.31 | 0 |
| RNFF1, 26 | 86 | 52 | 65 | 4.81 (0–19) | 1.88 | 12 |
| RNFF1, 41 | 98 | 71 | 89 | 10.20 (0.30) | 4.32 | 30 |
| rEFs; 5–9 | #97±1.80 | 72±5.60 | *#31±6.20 | 2.76±0.77 | *0.40±0.13 | *1.75±1.03 |
| rEFs; 23–29 | 87±5.90 | 64±2.0 | *58±8.90 | 3.77±0.44 | *1.52±0.20 | *11.0±0.70 |
| rESCs | ||||||
| RES6; 10 | 92 | 92 | 29 | 0.90 (0–5) | 0.04 | 1 |
| RES27; 10 | 82 | 67 | 42 | 1.12 (0–5) | 0.18 | 2 |
| RES27; 42 | 100 | 41 | 97 | 14.41 (1–27) | 5.47 | 52 |
| RES28; 9 | 77 | 62 | 37 | 0.72 (0–3) | 0.20 | 5 |
| RES28; 28 | 96 | 77 | 84 | 3.55 (0–9) | 0.51 | 9 |
| rESCs; 9–10 | #84±3.80 | 74±8.0 | 36±3.30 | ##0.91±0.l0 | 0.14±0.04 | 2.7±1.04 |
| riPSCs | ||||||
| NF13; 6 | 90 | 75 | 43 | 3.05 (0–13) | 0.89 | 2 |
| NF13; 15 | 100 | 56 | 88 | 12.36 (1–22) | 3.59 | 33 |
| QV28; 4 | 96 | 73 | 66 | 3.77 (0–15) | 1.94 | 12 |
| QV28; 15 | 96 | 57 | 69 | 4,96 (0–25) | 2.12 | 13 |
| MR39; 6 | 78 | 73 | 57 | 1.77 (0–5) | 0.39 | 9 |
| MR39, 22 | 91 | 67 | 89 | 7.97 (0–43) | 3.17 | 31 |
| riPSCs, 4–6 | 88±5.30 | **74±0.70 | **#55±6.70 | ##2.86±0.58 | **1.07±0.46 | 7.7±2.97 |
| riPSCs, 15–22 | 95±2.60 | **60±3.50 | **82±6.50 | 8.43±2.15 | **2.96±0.43 | 25.7±6.37 |
* and ** – mark indicators differing significantly (P≥0.95) on early stages and prolonged cultivation of rEFs and riPSCs, correspondingly. # – marks indicators differing significantly (P≥0.95) on early stages of cultivation of rEFs and rESCs, rEFs and riPSCs also, correspondingly. ## – marks indicators differing significantly (P≥0.95) on early stages of cultivation of rESCs and riPSCs. The results are presented as mean ± SD.