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. 2019 Aug 6;9:11372. doi: 10.1038/s41598-019-47862-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Adaptive RT-PCR amplification curves of 500 copies of Zika (A), dengue (B), and chikungunya (C) genomic RNA alone (blue curves), with 500 copies of genomic RNA from another virus present (magenta and orange curves), and with 500 copies of genomic RNA from both other viruses present (yellow curve). Amplification curves with non-target RNAs added (green and grey) are also included. Each assay contains the same reaction mixture including all three primer and probe sets and L-DNA probes. Each amplification curve is the average of three independent runs.