Table 1.

Oligonucleotide sequences used in these studies.

D-DNA Oligonucleotides	Zika forward primer	5′-CAGCTGGCATCATGAAGAAYC
	Zika reverse primer	5′-CACCTGTCCCATCTTTTTCTCC
	Zika hydrolysis probe	5′-FAM-CYGTTGTGG-ZEN-ATGGAATAGTGG-IABkFQ
	Dengue forward primer	5′-CAAAAGGAAGTCGYGCAATA
	Dengue reverse primer	5′-CTGAGTGAATTCTCTCTGCTRAAC
	Dengue hydrolysis probe	5′-HEX-CATGTGGYT-ZEN-GGGAGCRCGC-IABkFQ
	Chikungunya forward primer	5′-TACAGGGCTCATACCGCATC
	Chikungunya reverse primer	5′-AAAGGTGTCCAGGCTGAAGA
	Chikungunya hydrolysis probe	5′-Cy5-CGACCATGC-TOA-CGTCACAGTTAAGGA-IAbRQSp
L-DNA Oligonucleotides	Reverse transcription beacon	5′-FAM-GCGAGAAAAAAAAAAAAAAACTCGC-BHQ1
	Primer anneal probe	5′-TEX-CAGCTGGCATCATGAAGAATC
	Primer anneal compliment	5′-GATTCTTCATGATGCCAGCTG-BHQ2
	Target melt probe	5′-TEX-CTTTGTCACCGACGCCTACGTCGCAGGATCCTGGGCT GGCGGGTCGCTTCCACGATGGCCACCTCCATGGTCCTCGA
	Target melt compliment	5′-TCGAGGACCATGGAGGTGGCCATCGTGGAAGCGACCCGCC AGCCCAGGATCCTGCGACGTAGGCGTCGGTGACAAAG-BHQ2