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. 2019 Aug 6;9:11405. doi: 10.1038/s41598-019-47878-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Chlamydia Infectivity and gross pathology in female mice housed under LD conditions. Mice (n = 12 per group) were infected with C. muridarum at either ZT3 or ZT15. Experiment was repeated twice. (A) Mice infected at ZT3 had a significantly higher bacterial burden compared to mice infected at ZT15 from days 12 and 24 (***p < 0.001). Data was analysed using two-way analysis of variance (ANOVA). (B) Gross pathology in female mice housed under LD conditions. Note, mice infected ZT3 had paraovarian (next to the ovary) cysts as indicated by the white arrow, mice infected at ZT15 did not have paraovarian cysts.