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. 2019 Aug 6;9:11405. doi: 10.1038/s41598-019-47878-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Anti-chlamydia antibody determination in mice housed under LD conditions. Mice were infected with C. muridarum at either ZT3 or ZT15 and serum samples were collected at different time points after infection. (A) IgG was significantly higher in mice infected at ZT15 in the first week of infection compared to mice infected at ZT3 (*p < 0.05). However, during weeks two and three of infection, IgG was significantly higher in mice infected at ZT3 compared to mice infected at ZT15 (**p < 0.01). (B) IgG2C was significantly higher in mice infected at ZT3 compared to mice infected at ZT15 (**p < 0.01, ****p < 0.0001). (C) No significant difference in IgA levels between mice infected at ZT3 or ZT15. (D) Significant increase in IgA levels in mice infected at ZT15 in the fourth week of infection compared to mice infected at ZT3 (**p < 0.01). The data was analysed using a one-way ANOVA and post hoc test.