Fig. 9.

Angptl8 knockdown dampens the liver clock reset by food. Mice were transduced with AAVs carrying either Angptl8 shRNA or scramble shRNA for 45 days. a Serum Angptl8 levels in mice subjected to 12-h food deprivation (ZT13-1) or 12-h food deprivation followed by 20 min refeeding. n = 5, **P < 0.01 Refed vs. Fasted mice, ^##P < 0.01 AAV-Angpptl8 shRNA vs. AAV-scramble shRNA, one-way ANOVA followed by Bonferroni’s post hoc test. b, c qPCR analyses of Per1 and Per2 mRNA expression in the liver of mice from a. n = 5, **P < 0.01 Refed vs. Fasted mice. ^##P < 0.01 AAV-Angpptl8 shRNA vs. AAV-scramble shRNA, one-way ANOVA followed by Bonferroni’s post hoc test. d A schematic diagram illustrating the time design of DD and a fasting/refeeding cycle for liver-specific Angptl8 knockdown mice. e, f Time-course mRNA and serum levels of Angptl8 in mice subjected to 7-day DD and fasted for 12 h at CT13 or12-h food deprivation followed by refeeding for 21 h. g qPCR analyses of clock genes. h Serum levels of certain metabolites. For e–h n = 3, *P < 0.05 and **P < 0.01 refed vs. fasted mice. ^#P < 0.05 and ^##P < 0.01 AAV-Angpptl8 shRNA vs. AAV-scramble shRNA, two-way ANOVA followed by Bonferroni’s post hoc test. All data are presented as the means ± SD. Source data are provided as a Source Data file