Fig. 4.

Biodistribution of bacteria in tumor-bearing mice. 4T1 tumor-bearing mice with tumor volume around 50 mm³ were intravenously injected with EcN or CMCB (1 × 10⁷ CFUs) and then sacrificed at indicated time points. Organ homogenates were diluted and cultured on LB agar plates at 37 °C for 24 h prior to enumeration. Biodistribution at a 1 h, b 3 h, c 3 days, d 5 days, e 8 days, f 12 days post-injection, respectively. g The relationship between time post-injection and bacterial number within normal organs and tumor. Error bars represent the standard deviation (n = 5). Significance was assessed using Student’s t-test, giving p-values, *p < 0.05, **p < 0.01, ***p < 0.005. NS no significance