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. 2019 Aug 6;9:11359. doi: 10.1038/s41598-019-47837-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene expression profiling of BxPC3 CSCs treated with PRP using RNA Human EMT and CSCs RT2 Profiler PCR Array. BxPC3 CSCs were treated with PRP on day 2 and on day 4. On day 5, total RNA from treated and non-treated CSCs was extracted. A pool of three total RNAs extracted from three independent experiments was used for first strand cDNA synthesis. Gene expression was determined using RT² Profiler PCR Array of EMT and CSCs. (A) Expression of genes up regulated during EMT. (B) Expression of genes down regulated during EMT. (C) Expression of genes related to differentiation and development. (D) Expression of genes related to CSCs. All gene expressions were normalised to the untreated control.