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. 2019 Aug 6;9:11360. doi: 10.1038/s41598-019-47851-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Delayed effects of apical hyperosmolar media on IL-8 secretion by Caco-2 cells. Secreted IL-8 after 24-h apical hyperosmotic stress was detected by ELISA test on culture media obtained from undifferentiated Caco-2 cells grown for 3 days, or from media recovered at the basal side of differentiated Caco-2 monolayer after 15 days. Values are from three independent experiments (n = 3 for each experimental group). Mean significant differences (P < 0.05) are indicated by a different letter. Main factor and interaction effects are indicated with *P < 0.05, **P < 0.01 and ***P < 0.001. NS: Non-significant difference.