Table 1.
Effects of apical hyperosmolarity on expression of genes encoding for proteins involved in mitochondrial energy metabolism in Caco-2 monolayer.
| 24-h stress on proliferative cells | 24-h stress on differentiated cells | Statistical effects | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Gene | iso | 50% hyper | 100% hyper | iso | 50% hyper | 100% hyper | Stage | Osmotic | Stage x Osmotic |
| NDUFS1 | 1.00 ± 0.04 (a) | 0.92 ± 0.02 (a) | 1.45 ± 0.08 (b) | 1.00 ± 0.03 (a) | 1.28 ± 0.04 (b) | 1.24 ± 0.05 (b) | NS | *** | *** |
| SDHD | 1.08 ± 0.19 (a) | 1.03 ± 0.10 (a) | 1.71 ± 0.14 (b) | 1.01 ± 0.05 | 1.26 ± 0.06 | 1.33 ± 0.12 | NS | *** | NS |
| UQCRB | 1.01 ± 0.05 (a) | 0.85 ± 0.02 (a) | 1.42 ± 0.07 (b) | 1.00 ± 0.04 | 1.15 ± 0.02 | 1.17 ± 0.04 | NS | *** | *** |
| COX5B | 1.01 ± 0.07 | 0.95 ± 0.04 | 1.04 ± 0.02 | 1.00 ± 0.03 | 1.07 ± 0.05 | 1.13 ± 0.02 | NS | NS | NS |
| ATP5F1B | 1.02 ± 0.09 | 0.88 ± 0.05 | 0.89 ± 0.05 | 1.00 ± 0.04 (a) | 1.32 ± 0.08 (b) | 1.31 ± 0.06 (b) | *** | NS | *** |
| CS | 1.00 ± 0.03 | 1.28 ± 0.04 | 1.24 ± 0.05 | 1.00 ± 0.04 | 1.08 ± 0.05 | 1.05 ± 0.03 | * | NS | * |
Relative gene expressions were measured in proliferative (7 days after seeding) and differentiated (15 days after seeding) Caco-2 cells after 24-h of osmotic treatments. Values are mean ± s.e.m. from three independent experiments each in duplicate (n = 6). Mean significant differences (P < 0.05) are indicated by a different letter. Main factor and interaction effects are indicated with *P < 0.05, **P < 0.01 and ***P < 0.001. NS: Non-significant difference.