Table 3.
Proliferative cells (3 to 7 days after cell seeding) | Differentiated cells (15 days after cell seeding) | |
---|---|---|
2-h tests: | Cell viability and proliferation | Gene expression |
Oxygen consumption | TER and markers of epithelial permeability | |
24-h tests: | Oxygen consumption | Gene expressions |
Mitochondrial gene expressions | TER and markers of epithelial permeability | |
IL8 secretion | IL8 secretion |
Proliferative Caco-2 cells (3 or 7 days post-seeding on plates or porous filters) were used before confluence. Differentiated Caco-2 monolayers (15 days post-seeding on porous filters) had a TER > 300 ohm*cm2.