Table 4.
List of primers used in gene expression studies.
| Protein name (Gene symbol) | Upper primer (5′ → 3′) | Lower primer (5′ → 3′) |
|---|---|---|
| Occludin (OCLN) | ACCCCCATCTGACTATGTG | CTTGCTCTGTTCTCTTTGACC |
| F11 receptor (JAMA) | CACCACCAGACTCGTTTG | GCCTTCCTCAGAGACCATAC |
| Zona occludens Protein 1 (TJP1) | CCGTGTTGTGGATACCTTG | GCCTGCTGTTTTTGGAG |
| Claudin-1 (CLDN1) | TGAGGATGGCTGTCATTG | GGTAAGAGGTTGTTTTTCGG |
| Claudin-2 (CLDN2) | AGCAGCCCAGACAATGAG | TAGGATGTAGCCCACAAGTTG |
| Claudin-5 (CLDN5) | TGGGTCACTGGGAACTTC | AGTCTCTGGCAAAAAGCG |
| Na+/K+-ATPase alpha subunit (ATP1A1) | GGAGACGAGGAACATTGC | GACACACCCAGGAACACAG |
| Na+/H+ type 1 exchanger (NHE1) | GGTTCTGGCTGTCTTTGAG | ATGTGGGAGGTAAATCGG |
| Na+/H+ type 3 exchanger (NHE3) | TGACGCTGGTCTTCATCTC | GTGCTCGCTCCTCTTCAC |
| Na+/K+/2Cl− type 1 co-transporter (NKCC1) | TACCCACACCAACACCTACTAC | CACGACTCCTTTACTTTCTGC |
| Aquaporin 3 (AQP3) | CTTCTTGGGTGCTGGAATAG | TGCCTATGAACTGGTCAAAG |
| ATP synthase (ATP5F1B) | CATCTCCTTCGCCAAAAG | TGATTCTGCCCAAAGTCTC |
| Cytochrome C oxidase 5A (COX5) | CGCTGGGTAACATACTTCAAC | GATGACATAGGGGTAGATTTCC |
| Ubiquinol cytochrome C reductase binding protein (UQCRB) | AAGGCAACGCTTCTCTTTC | CCCCAGTTTATTGAATCCTG |
| NADH Ubiquinone oxidoreductase S1 (NDUFS1) | GCAGGAGTAGATGATTTGGG | GGCATAGGGCTTAGAGGTTAG |
| Succinate deshydrogenase (SDHD) | ACCGACCTATCCCAGAATG | CTGAAAGTGCCAAAAGCC |
| Citrate synthase (CS) | TTGGCTGACCTGATACCTAAG | CAAGATACCTGTTCCTCTGTTG |
| Cyclooxygenase 2 (PTGS2) | TCAGCCATACAGCAAATCC | GGTGTTGAGCAGTTTTCTCC |
| Nuclear factor in activated T cell 5 (NFAT5) | GGACATTGAAGGCACTACTG | TTGGAGAAGAGGGTGTTTG |
| Interleukin 6 (IL6) | CTGAACCTTCCAAAGATGGC | AGCAGGCTGGCATTTGTGGT |
| Interleukin 8 (CXCL8) | TCTCAGCCCTCTTCAAAAACTTCTC | ATGACTTCCAAGCTGGCCGTGGCT |
| Hypoxanthine Phosphoribosyltransferase 1 (HPRT) | ATGTTGCGTTGGAAGTGTAG | AGGTATGCGGTATTTGGC |
| 60S ribosomal protein L18 (RPL18) | ACCTGGCCGAGCAGGAG | TGGAGTTGGTTCTTCTGGC |