Fig. 5.
Thanatin inactivates NDM-1. a Thanatin induced NDM-1 release to E. coli culture supernatant. b Hydrolytic effects of the supernatant on imipenem. The supernatant was obtained from thanatin-treated NDM-1-producing E. coli XJ141026 at 6 h. c Kd value for binding of thanatin to RED-tris-NTA-labeled apo-NDM-1 was obtained by MST assay. d Thanatin inhibited the activity of NDM-1. e The Lineweaver–Burk plot. Thanatin is a competitive inhibitor of NDM-1. f Molar equivalents of zinc in holo-NDM-1 were detected by ICP-MS after thanatin treatment. Data are shown as the mean ± s.e.m. from three independent experiments. P-values were determined by two-way ANOVA (a, b), or one-way ANOVA with Bonferroni’s comparison test (f). *P < 0.05 **P < 0.01, ***P < 0.001 vs. control. Source data are provided in Source Data file