Fig. 6.

Thanatin rescues the activity of carbapenem. a Microdilution checkerboard analysis showing the combined effect of thanatin (Tha) and meropenem (Mem) against NDM-1-producing E. coli XJ141026. The heat plot showed an average of three technical replicates. b, c Sub-MICs of thanatin reversed the activity of meropenem (b) and imipenem (Imp) (c) resistance in vitro. d NDM-1-producing E. coli XJ141026-infected mice were treated with a single dose of meropenem (10 mg/kg), a combination of meropenem (10 mg/kg) and thanatin (0.1 mg/kg), thanatin alone (0.1 mg/kg), or PBS via intraperitoneal injection (n = 14 per group). e, f Bacterial loads in the spleen (e) and liver (f) of thanatin-treated E. coli XJ141026-infected mice were determined by plating the samples for colony counts 24 h after treatment (n = 6 per group). Data are shown as the mean ± s.e.m. from at least three independent experiments. P-values were determined by one-way ANOVA with Bonferroni’s comparison test (b, c, e, f), or by log-rank test (d). **P < 0.01, ***P < 0.001; ^##P < 0.01 between 10 mg/kg Mem and Tha + Mem. Source data are provided in Source Data file