Fig. 5.

Burst size of Pan/99 virus is constant over a range of high MOIs. a MDCK cells were inoculated with Pan/99-WT virus at MOIs of 1, 3, 6, 10, and 20 PFU/cell under single-cycle conditions. Infectious titers at each time point are shown, with MOI indicated by the colors defined in the legend. Dashed line indicates the limit of detection (50 PFU/mL). b Fraction of cells expressing HA at each MOI, as measured by flow cytometry staining of cells 12 h post inoculation. c Burst size in PFU produced per HA⁺ cell, with individual data points overlaid on bars. Text indicates mean ± SD estimate of burst size. In all panels, mean and standard error (N = 3 replicates per MOI) are plotted, and colors correspond to the legend in panel a. Source data are provided as a Source Data file