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. 2019 Aug 6;9:11381. doi: 10.1038/s41598-019-47916-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of T-DNA insertional atabcg1 mutant lines. (A) Genomic organization of the two independent T-DNA insertional atabcg1 mutant lines, atabcg1-3 and atabcg1-4. The atabcg1-3 line contains a T-DNA insertion at 791 bp and the atabcg1-4 line one at 996 bp. Arrows indicate the primers used for the mutant characterization. (B) Genetic analysis of the atabcg1 mutant lines atabcg1-3 and atabcg1-4. Genomic DNA of wildtype (WT) and mutant lines (#1) were analyzed for AtABCG1 gene specific, T-DNA specific and actin specific amplification products. N, negative control. (C) Presence of full-length AtABCG1 transcripts in T-DNA insertional atabcg1 mutant lines. Amplification of full-length AtABCG1 was performed from cDNA isolated from atabcg1-3 and atabcg1-4 lines and the depicted primer pairs. Entire gels can be found in supplemental information (Figs S5 and S6).