Fig. 6.

In vitro fibrinolytic activity of purified enzyme. a Spontaneous blood clot in four catheter tubes. b The effect of 100 U, 50 U, and 10 U of purified enzyme on blood clot in tubes 1, 2, and 3, respectively, after 5 h incubation. Normal saline was added to tube 4. Tube 1 shows complete dissolution of blood clot, while 2, 3 show partial degradation and 4 shows intact clot. (c) In vitro degradation after 18 h incubation. Tubes 1, 2 show complete clot lysis and 3 shows incomplete clot lysis while 4 shows intact clot. (d) Fibrinolytic activity of 1 U purified enzyme and plasmin on fibrin plate after Congo red staining. The diameters of the hydrolyzed clear zone were measured and compared