Table 2.
Experimental design and the response of central composite design (CCD) and artificial neural network (ANN)
| Run | Wheat bran extract* | Yeast extract* | CaCl2* | Enzyme Activity (U/mL) | ||
|---|---|---|---|---|---|---|
| X 1 | X 2 | X 3 | Experimental | RSM predicted | ANN predicted | |
| 1 | 0 (35) | 0 (15) | 0 (3) | 4172.13 | 4130.11 | 4172.11 |
| 2 | − 1 (20) | 1 (20) | − 1 (2) | 3639.34 | 3649.92 | 3639.31 |
| 3 | 0 (35) | 0 (15) | 0 (3) | 4172.13 | 4130.11 | – |
| 4 | 0 (35) | − 1.68 (6.59) | 0 (3) | 2483.61 | 2533.29 | 2483.59 |
| 5 | 1 (50) | − 1 (10) | − 1 (2) | 2909.84 | 2896.20 | 2909.83 |
| 6 | 0 (35) | 0 (15) | 0 (3) | 4114.75 | 4130.11 | – |
| 7 | − 1 (20) | − 1 (10) | − 1 (2) | 2655.74 | 2673.42 | 2655.89 |
| 8 | − 1.68 (9.77) | 0 (15) | 0 (3) | 3368.85 | 3390.17 | 3368.83 |
| 9 | 0 (35) | 0 (15) | 0 (3) | 4122.95 | 4130.11 | – |
| 10 | 0 (35) | 1.68 (23.41) | 0 (3) | 3713.11 | 3727.62 | 3712.86 |
| 11 | 1.68 (60.23) | 0 (15) | 0 (3) | 3508.2 | 3551.17 | 3508.20 |
| 12 | 1 (50) | − 1 (10) | 1 (4) | 3327.87 | 3270.00 | 3327.85 |
| 13 | − 1 (20) | 1 (20) | 1 (4) | 3778.69 | 3745.12 | 3778.67 |
| 14 | 0 (35) | 0 (15) | − 1.68 (0.07) | 3606.56 | 3560.41 | 3606.54 |
| 15 | 0 (35) | 0 (15) | 1.68 | 3844.26 | 3954.79 | 3844.24 |
| 16 | 0 (35) | 0 (15) | 0 (3) | 4040.98 | 4130.11 | – |
| 17 | 1 (50) | 1 (20) | − 1 (2) | 3934.43 | 3958.80 | 3942.18 |
| 18 | 0 (35) | 0 (15) | 0 (3) | 4172.13 | 4130.11 | – |
| 19 | − 1 (20) | − 1 (10) | 1 (4) | 3459.02 | 3387.42 | 3459.01 |
| 20 | 1 (50) | 1 (20) | 1 (4) | 3778.69 | 3713.80 | 3778.67 |
*Uncoded values (g/L) are shown in parentheses