Table 7.
Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Purification fold | |
---|---|---|---|---|
Crude | 31,55,738 | 2040.7 | 1546.41 | |
Ammonium sulfate precipitation | 30,57,377 | 325.0 | 9407.31 | 6.1 |
DEAE A-50 | 1,960,656 | 22.0 | 89,120.72 | 57.6 |
Ultracentrifugation | 885,246 | 8.0 | 110,655.74 | 71.6 |
Sephacryl S-100 HR | 475,410 | 3.8 | 125,107.85 | 81 |