Table 1.
Assays of growth, sporulation, and pathogenicity of the MoCDIPs overexpression and knock out mutants
| Gene ID | Mutant | Vegetative growth (cm) |
Sporulation (104 spores·mL− 1) |
Pathogenicity | |
|---|---|---|---|---|---|
| Barley lesion (cm)a | Rice seedling (Disease level)b | ||||
| Guy11 | 6.59 ± 0.21 | 45.72 ± 6.48 | 6.71 ± 0.69 | 4–5 | |
| MGG_01532 | OE-MoCDIP6 | 5.22 ± 0.08** | 46.38 ± 7.36 | 6.25 ± 0.66 | 4–5 |
| Δ-MoCDIP6 | 6.60 ± 0.19 | 45.22 ± 8.50 | 7.05 ± 0.57 | 4–5 | |
| MGG_03354 | OE-MoCDIP7 | 6.62 ± 0.14 | 46.60 ± 6.43 | 6.83 ± 0.58 | 4–5 |
| Δ-MoCDIP7 | 6.48 ± 0.17 | 39.22 ± 5.37 | 7.10 ± 0.70 | 4–5 | |
| MGG_05038 | OE-MoCDIP8 | 6.59 ± 0.28 | 43.33 ± 4.97 | 6.13 ± 0.80 | 4–5 |
| Δ-MoCDIP8 | 6.83 ± 0.15 | 49.53 ± 6.62 | 6.42 ± 0.42 | 4–5 | |
| MGG_08411 | OE-MoCDIP9 | 6.40 ± 0.07 | 47.23 ± 8.73 | 6.50 ± 0.48 | 4–5 |
| ND | ND | ND | ND | ND | |
| MGG_12275 | OE-MoCDIP10 | 6.55 ± 0.47 | 48.97 ± 6.89 | 6.88 ± 0.31 | 4–5 |
| Δ-MoCDIP10 | 6.65 ± 0.18 | 53.47 ± 7.80 | 6.63 ± 0.48 | 4–5 | |
| MGG_12521 | OE-MoCDIP11 | 6.53 ± 0.19 | 43.10 ± 5.13 | 6.75 ± 0.50 | 4–5 |
| Δ-MoCDIP11 | 6.66 ± 0.13 | 51.58 ± 6.21 | 6.58 ± 0.30 | 4–5 | |
| MGG_13283 | OE-MoCDIP12 | 6.51 ± 0.23 | 40.27 ± 6.90 | 6.21 ± 0.72 | 4–5 |
| ND | ND | ND | ND | ND | |
| MGG_14371 | OE-MoCDIP13 | 6.58 ± 0.09 | 39.72 ± 5.61 | 7.05 ± 0.50 | 4–5 |
| ND | ND | ND | ND | ND | |
aInfection on detached barley leaves was assessed by measuring the diameter of the lesions on the leaves;
bDisease severity was scored by following 0–5 scale (0–1: Resistant, 2: moderately resistant, 3: moderately susceptible, 4–5: severe susceptible);
Statistical significances (*P < 0.05 or **P < 0.01) between the MoCDIPs mutants and the wild type Guy11 were analyzed by student’s t-test; ND Not determined